This product is a 74.4 kDa purified recombinant protein inducibly expressed in E. coli carrying the φ29 DNA polymerase gene. With high sensitivity and great synthesis ability, it can be used for whole genome amplification from a single cell. It possesses 3'→5' proofreading exonuclease activity to ensure high fidelity of resulting DNA. Its properties of multiple strand displacement and processive synthesis allow >10 kb lengths of amplification products from genome or plasmid DNA, and enable rolling-circle replication of circular DNA.

Highlights

· Isothermal amplification;

· High fidelity; high efficiency; high sensitivity; high yield;

· Random primers or specific N9 primers can be used.

Application

Sequencing, SNP genotyping, STR/ microsatellite analysis, virus detection, miRNA detection, etc.

Storage
at -20°C for two years

Shipping
Dry ice (-70 ℃)

Component	LP101-01	LP101-02
Phi29 DNA Polymerase	250 units	5×250 units
10×Phi29 DNA Polymerase Buffer	50 μl	250 μl
Phi29 Random Primers	125 μl	625 μl
10 mM dNTPs	25 μl	125 μl
10×DNA Loading Buffer	1 ml	1 ml