TransStart® FastPfu Fly DNA Polymerase is a hot start, high-fidelity and high processivity DNA Polymerase used for fast PCR. Compared with TransStart® FastPfu DNA Polymerase, TransStart® FastPfu Fly DNA Polymerase has higher extension rate (6kb/min vs 4kb/min), featuring higher amplification efficiency, higher yield, higher fidelity and higher specificity.2×TransStart® FastPfu Fly Reaction Mix already contains dNTPs. When DNA is amplified, just add template, primer, water and TransStart® FastPfu Fly DNA Polymerase. The Reaction can be carried out with the concentration of Reaction Mix being 1×. PCR products are blunt end and can be cloned directly into pEASY®-Blunt series of vectors.

• Offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.

• PCR products can be directly cloned into pEASY®-Blunt vectors.

• Amplification of genomic DNA fragment up to 15 kb.

• Amplification of plasmid DNA fragment up to 20 kb.

Applications

• Hot start, high specificity.

• High amplification efficiency.

• Fast and high fidelity.

• High sensitivity.

• High production.

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)

Product Contents

Component	AP231-21-V3	AP231-22-V3	AP231-23-V3
TransStart^® FastPfu Fly DNA Polymerase	250 U×1	500 U×1	500 U×6
2×TransStart^® FastPfu Fly Reaction	1 ml×3	1 ml ×6	1 ml ×36
50 mM MgSO₄	200 μl×1	400 μl ×1	1 ml ×1
PCR Stimulant	200 μl×1	400 μl×1	1 ml ×1
6×DNA Loading Buffer	500 μl×1	1 ml ×1	1 ml ×2

Citations

Zhu X J , Sun S , Xie B , et al. Guanine-rich sequences inhibit proofreading DNA polymerases[J]. Scientific Reports, 2016, 6:28769.

Ye F , Lan X E , Zhu W B , et al. Mitochondrial genomes of praying mantises (Dictyoptera, Mantodea): rearrangement, duplication, and reassignment of tRNA genes[J]. Scientific Reports, 2016, 6:25634.

Hu L , Li H , Qin R , et al. Plant phosphomannose isomerase as a selectable marker for rice transformation[J]. Scientific Reports, 2016, 6(1):25921.

Chao H , Zhou N Y . GenR, an IclR-Type Regulator, Activates and Represses the Transcription of gen Genes Involved in 3-Hydroxybenzoate and Gentisate Catabolism in Corynebacterium glutamicum[J]. Journal of Bacteriology, 2013, 195(7):1598-1609.

Liu X, Pu J, Zeng S, et al. Hyriopsis cumingii Hic52-A novel nacreous layer matrix protein with a collagen-like structure[J].Int J Biol Macromol. 2017 Sep;102:667-673.

Hot Start PCR

TransStart® FastPfu Fly DNA Polymerase