VAHTS TGS DNA Library Prep Kit for Oxford Nanopore Technologies ONT

Size: 24 Rxns.

Keywords: DNA library construction; third generation library construction; TGS library construction; Nanopore sequencing; ONT sequencing

VAHTS TGS DNA Library Prep Kit for ONT is a ligation-based library construction kit suitable for Nanopore sequencing platform. Combined with the Barcode Adapter, it can perform single-sample or multi-sample analysis of 100 ng - 1.5 μg gDNA and amplicons. Build a database. This kit uses a directed evolution mutant enzyme with high catalytic activity and a carefully optimized buffer. It can directly perform Barcode Ligation without purification after end repair/damage repair/A-tail addition, and the experimental process is convenient. All reagents provided in the kit have undergone strict quality control and functional verification to ensure the stability and reproducibility of library construction.

Features:

1) The operation process is simple: steps of end repair + damage repair + adding A tail are completed in one tube, no purification is required, and Barcode connection can be performed directly

2) The time required for library construction is shortened: the minimum time required for the entire process of database construction can be <2 hours, which saves ≥30 minutes compared with the conventional process.

3) Compatible with various species you need to work with: compatible with animals, plants, microorganisms and other species

4) Excellent data quality: high read rate of long fragments, high data output, and good data quality

Better Data output and Quality

Species

Metric

TS201

Supplier A

Human

Clean Total Reads

1,910,636

1,754,451

Clean Total bases

37,568,318,787

30,769,214,717

Clean Mean quality(Mean Q)

14.77

14.69

Plant

Clean Total Reads

3,398,731

3,494,821

Clean Total bases

32,590,018,744

31,687,125,980

Clean Mean quality(Mean Q)

12.86

12.77

Bacteria

Clean Total Reads

223,825

287,405

Clean Total bases

1,629,142,727

1,791,295,356

Clean Mean quality(Mean Q)

15.5

15.3

Better Longer Reads

Species

DNA Size

TS201

Supplier A

Human

5 - 50 kb

63.63%

60.95%

50-100 kb

8.25%

6.57%

100-200 kb

0.13%

0.11%

Total

72.01%

67.63%

Plant

5 - 50 kb

39.63%

37.02%

50-100 kb

2.22%

2.10%

100-200 kb

0.02%

Total

41.87%

39.14%

Kit composition

Component	TS201-01 (24 rxns)
End Prep & Repair Buffer	36 µL
End Prep & Repair Master Enzyme Mix	36 µL
Barcode Rapid Ligase Mix	96 µ L
MP Adapter Fast Ligation Buffer	240 µL
MP Adapter Fast Ligase	120 µL